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KMID : 0861020210360020001
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Korea Journal of Herbology
2021 Volume.36 No. 2 p.1 ~ p.9
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Gossypii Semen oil alleviates memory dysfunction in scopolamine-treated mice
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Lee Ji-Hye
Jung Eun-Mi
Lee Eun-Hong
Jang Gwi-Yeong
Seo Kyung-Hye
Kim Mi-Ryeo
Jung Ji-Wook
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Abstract
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Objectives : Gossypium arboreum (cotton) is traditionally used to treat various health disorders. However, anti-amnesic effect of G. arboreum has not been reported. The objective of this study was to investigate in-vivo the anti-amnesic effects along with in vitro antioxidant and acetylcholinesterase (AChE) inhibition potential in G.
arboreum seed essential oil.
Methods : The essential oil of G. arboreum obtained by solid phase microextraction (SPME) techniques were identified by gas chromatography-mass spectroscopy (GC-MS). 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'- azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay were performed to determine the antioxidant activity at various concentrations (312.5, 625, 1250, 2500, 5000, 10000 ㎍/㎖. Y-maze, passive avoidance and Morris water maze tests were carried out to evaluate improved effect on scopolamine (1 ㎎/㎏)-induced memory dysfunction at the dose level of 50, 100 and 200 ㎎/㎏. Donepezil (5 ㎎/㎏) was used as a positive drug control. We performed acetylcholinesterase (AChE) activity assay in ex vivo.
Results : Five volatile compounds were identified in G. arboreum. The assays of DPPH and ABTS revealed that G.
arboreum increased antioxidant activity in a dose-dependent manner. G. arboreum ameliorated the percent of spontaneous alternation in the Y-maze test, shortened step-through latency in the passive avoidance test, and increased swimming time in the target zone in the Morris water maze test. In addition, G. arboreum inhibited the AChE activity.
Conclusions : Based on these findings, G. arboreum may aid in the prevention and treatment of learning and memory-deficit disorders through antioxidant and AChE inhibitory activities.
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KEYWORD
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Scopolamine, Memory, Essential oil, Cotton seed, Gossypii Semen
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